pET28-p53 (1-73) (72P)

62083 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
Expression of human p53 (1-73) (72P) in e. coli
Depositor
Gary Daughdrill
Article
Vise et al Nucleic Acids Res. 2005 Apr 11;33(7):206
Insert
p53 (TP53 Human)
Tags
HIs
Mutation
contains TAD residues 1-73; 72P
Promoter
T7
Available Since
Feb. 11, 2015
Availability
Available

Sequence Information

Length
785 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 3
Label Gene Product Position Strand
6xHis — 6xHis affinity tag 45..63 Fwd
DNA Sequence
CATCATCATCATCATCAC
Protein Sequence
HHHHHH
thrombin site — thrombin recognition and cleavage site 72..90 Fwd
DNA Sequence
CTGGTGCCGCGCGGCAGC
Protein Sequence
LVPRGS
6xHis — 6xHis affinity tag 324..342 Fwd
DNA Sequence
CACCACCACCACCACCAC
Protein Sequence
HHHHHH
terminator 1
Label Gene Product Position Strand
T7 terminator — — 408..456 Fwd
DNA Sequence
CTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG