Human PNP his-tagged

64076 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
Expression of human Purine Nucleoside Phosphorylase
Depositor
Vern Schramm
Article
Lewandowicz et al J Biol Chem. 2003 Aug 22;278(34):31465-8
Insert
Purine Nucleoside Phosphorylase (PNP Human)
Tags
Histidine tag
Available Since
April 29, 2015
Availability
Available

Sequence Information

Length
882 bp
Topology
linear
Molecule Type
genomic DNA

Features

CDS 3
Label Gene Product Position Strand
6xHis — 6xHis affinity tag 28..46 Fwd
DNA Sequence
CATCATCATCATCATCAT
Protein Sequence
HHHHHH
T7 tag (gene 10 leader) — leader peptide from bacteriophage T7 ge… 49..82 Fwd
DNA Sequence
ATGGCTAGCATGACTGGTGGACAGCAAATGGGT
Protein Sequence
MASMTGGQQMG
Xpress(TM) tag — Xpress(TM) epitope tag, including an en… 85..109 Fwd
DNA Sequence
GATCTGTACGACGATGACGATAAG
Protein Sequence
DLYDDDDK