HGXPRT

64201 Synthetic Bacterial Expression

Plasmid Information

Purpose
Expression of Plasmodium falciparum HGXPRT
Depositor
Vern Schramm
Article
Hazleton et al Chem Biol. 2012 Jun 22;19(6):721-30. doi
Insert
Hypoxanthine guanine xanthine phosphoribosyltransferase
Tags
Histidine tag
Available Since
April 29, 2015
Availability
Available

Sequence Information

Length
850 bp
Topology
linear
Molecule Type
genomic DNA

Features

CDS 2
Label Gene Product Position Strand
6xHis — 6xHis affinity tag 48..66 Fwd
DNA Sequence
CATCACCACCATCACCAC
Protein Sequence
HHHHHH
thrombin site — thrombin recognition and cleavage site 72..90 Fwd
DNA Sequence
CTGGTACCTCGCGGCTCT
Protein Sequence
LVPRGS
protein_bind 1
Label Gene Product Position Strand
attB2 mutant version of attB — 797..822 Rev
DNA Sequence
ACCACTTTGTACAAGAAAGCTGGGT