pJexpress414_OmpA171_R4cons_Y129H

64302 Synthetic Bacterial Expression

Plasmid Information

Purpose
Conservative version of OmpA Redesign 4 with Y129H mutation
Depositor
Timothy Whitehead
Article
Stapleton et al Proc Natl Acad Sci U S A. 2015 Jul 21. p
Insert
OmpA_R4cons_Y129H
Tags
6His and FlAsH
Mutation
25 mutations to lipid-facing surface
Promoter
T7
Available Since
Aug. 6, 2015
Availability
Available

Sequence Information

Length
833 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 2
Label Gene Product Position Strand
tetracysteine tag — tetracysteine peptide that binds biarse… 596..614 Fwd
DNA Sequence
TGTTGCCCTGGTTGCTGT
Protein Sequence
CCPGCC
6xHis — 6xHis affinity tag 614..632 Fwd
DNA Sequence
CACCATCACCACCATCAC
Protein Sequence
HHHHHH
sig_peptide 1
Label Gene Product Position Strand
OmpA signal peptide — signal peptide from the E. coli outer m… 20..83 Fwd
DNA Sequence
ATGAAAAAGACCGCAATCGCCATCGCAGTGGCACTGGCTGGTTTCGCTACCGTAGCGCAGGCC
terminator 1
Label Gene Product Position Strand
T7 terminator — — 645..693 Fwd
DNA Sequence
CTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG