4RCI

64653 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
Bacterial expression for structure determination; may not be full ORF
Depositor
Cheryl Arrowsmith
Insert
YTHDF1:JMC02A-E04:C231829 (YTHDF1 Human)
Tags
6xHis
Mutation
See comments
Promoter
T7
Available Since
April 29, 2015
Availability
Available

Sequence Information

Length
725 bp
Topology
linear
Molecule Type
genomic DNA

Features

CDS 3
Label Gene Product Position Strand
6xHis — 6xHis affinity tag 12..30 Fwd
DNA Sequence
CATCATCATCATCATCAC
Protein Sequence
HHHHHH
TEV site — tobacco etch virus (TEV) protease recog… 42..63 Fwd
DNA Sequence
GAAAACTTGTATTTCCAGGGC
Protein Sequence
ENLYFQG
6xHis — 6xHis affinity tag 689..707 Fwd
DNA Sequence
CACCACCACCACCACCAC
Protein Sequence
HHHHHH