| Label | Gene | Product | Position | Strand | |
|---|---|---|---|---|---|
| 6xHis | — | 6xHis affinity tag | 40..58 | Fwd | |
|
DNA Sequence
CATCATCATCATCATCAC
Protein Sequence
HHHHHH
|
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| thrombin site | — | thrombin recognition and cleavage site | 67..85 | Fwd | |
|
DNA Sequence
CTGGTGCCGCGCGGCAGC
Protein Sequence
LVPRGS
|
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| T7 tag (gene 10 leader) | — | leader peptide from bacteriophage T7 ge… | 88..121 | Fwd | |
|
DNA Sequence
ATGGCTAGCATGACTGGTGGACAGCAAATGGGT
Protein Sequence
MASMTGGQQMG
|
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| 6xHis | — | 6xHis affinity tag | 703..721 | Fwd | |
|
DNA Sequence
CACCACCACCACCACCAC
Protein Sequence
HHHHHH
|
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