pET15b+PCNA1 G108C/P186C

66174 Synthetic Bacterial Expression

Plasmid Information

Purpose
Expresses the G108C/P186C mutant of Sulfolobus solfataricus PCNA1
Depositor
Teruyuki Nagamune
Article
Hirakawa et al Biotechnol Bioeng. 2013 Jul;110(7):1858-
Insert
DNA polymerase sliding clamp 1
Tags
His6 tag
Mutation
G108C, P186C
Promoter
T7 promoter
Available Since
June 30, 2015
Availability
Available

Sequence Information

Length
974 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 2
Label Gene Product Position Strand
thrombin site — thrombin recognition and cleavage site 776..794 Rev
DNA Sequence
CTGGTGCCGCGCGGCAGC
Protein Sequence
LVPRGS
6xHis — 6xHis affinity tag 803..821 Rev
DNA Sequence
CATCATCATCATCATCAC
Protein Sequence
HHHHHH
promoter 1
Label Gene Product Position Strand
T7 promoter — — 901..920 Rev
DNA Sequence
TAATACGACTCACTATAGG
protein_bind 1
Label Gene Product Position Strand
lac operator — — 876..901 Fwd
DNA Sequence
GGAATTGTTATCCGCTCACAATTCC