pET15b+PCNA2 WT

66175 Synthetic Bacterial Expression

Plasmid Information

Purpose
Expresses the wild type of Sulfolobus solfataricus PCNA2
Depositor
Teruyuki Nagamune
Article
Hirakawa et al Biotechnol Bioeng. 2013 Jul;110(7):1858-
Insert
DNA polymerase sliding clamp 2
Tags
His6 tag
Promoter
T7 promoter
Available Since
June 30, 2015
Availability
Available

Sequence Information

Length
948 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 2
Label Gene Product Position Strand
thrombin site — thrombin recognition and cleavage site 767..785 Rev
DNA Sequence
CTGGTGCCGCGCGGCAGC
Protein Sequence
LVPRGS
6xHis — 6xHis affinity tag 794..812 Rev
DNA Sequence
CATCATCATCATCATCAC
Protein Sequence
HHHHHH
promoter 1
Label Gene Product Position Strand
T7 promoter — — 892..911 Rev
DNA Sequence
TAATACGACTCACTATAGG
protein_bind 1
Label Gene Product Position Strand
lac operator — — 867..892 Fwd
DNA Sequence
GGAATTGTTATCCGCTCACAATTCC