pET15b+PCNA3 R112C/T180C

66182 Synthetic Bacterial Expression

Plasmid Information

Purpose
Expresses the R112C/T180C mutant of Sulfolobus solfataricus PCNA3
Depositor
Teruyuki Nagamune
Article
Hirakawa et al Biotechnol Bioeng. 2013 Jul;110(7):1858-
Insert
DNA polymerase sliding clamp 3
Tags
His6 tag
Mutation
R112C, T180C
Promoter
T7 promoter
Available Since
June 30, 2015
Availability
Available

Sequence Information

Length
927 bp
Topology
linear
Molecule Type
genomic DNA

Features

CDS 2
Label Gene Product Position Strand
thrombin site — thrombin recognition and cleavage site 805..823 Rev
DNA Sequence
CTGGTGCCGCGCGGCAGC
Protein Sequence
LVPRGS
6xHis — 6xHis affinity tag 832..850 Rev
DNA Sequence
CATCATCATCATCATCAC
Protein Sequence
HHHHHH
protein_bind 1
Label Gene Product Position Strand
lac operator — — 909..926 Fwd
DNA Sequence
TTGTTATCCGCTCACAA