pOPINK-TRABID (NZF1-3, aa 1-178)

66185 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
Expresses TRABID NZF1-3 (aa 1-178) in E. coli
Depositor
David Komander
Article
Michel et al Mol Cell. 2015 Apr 2;58(1):95-109. doi:
Insert
TRABID (ZRANB1 Human)
Tags
His6-GST
Mutation
only codes for amino acids 1-178 of TRABID
Promoter
T7
Available Since
July 17, 2015
Availability
Available

Sequence Information

Length
726 bp
Topology
linear
Molecule Type
genomic DNA

Features

CDS 2
Label Gene Product Position Strand
6xHis — 6xHis affinity tag 15..33 Rev
DNA Sequence
CACCACCACCACCACCAC
Protein Sequence
HHHHHH
HRV 3C site — recognition and cleavage site for human… 590..614 Rev
DNA Sequence
CTGGAAGTTCTGTTTCAGGGCCCG
Protein Sequence
LEVLFQGP