pRSET-triple mutation-pa-GCaMP6f

67570 Synthetic Bacterial Expression

Plasmid Information

Purpose
bacterial expression of a photoactivatable fluorescent calcium reporter
Depositor
Ehud Isacoff
Article
Berlin et al Nat Methods. 2015 Jul 13. doi: 10.1038/n
Insert
pa-GCaMP6f
Tags
HIS-tag
Mutation
V115H, L64F, T65S
Promoter
T7
Available Since
Aug. 6, 2015
Availability
Available

Sequence Information

Length
786 bp
Topology
linear
Molecule Type
genomic DNA

Features

CDS 4
Label Gene Product Position Strand
6xHis — 6xHis affinity tag 39..57 Fwd
DNA Sequence
CATCATCATCATCATCAT
Protein Sequence
HHHHHH
T7 tag (gene 10 leader) — leader peptide from bacteriophage T7 ge… 60..93 Fwd
DNA Sequence
ATGGCTAGCATGACTGGTGGACAGCAAATGGGT
Protein Sequence
MASMTGGQQMG
Xpress(TM) tag — Xpress(TM) epitope tag, including an en… 96..120 Fwd
DNA Sequence
GATCTGTACGACGATGACGATAAG
Protein Sequence
DLYDDDDK
calmodulin-binding peptide — calmodulin-binding peptide from avian s… 147..204 Fwd
DNA Sequence
CGTCGTAAGTGGAATAAGACAGGTCACGCAGTCAGAGCTATAGGTCGGCTGAGCTCA
Protein Sequence
RRKWNKTGHAVRAIGRLSS