| Label | Gene | Product | Position | Strand | |
|---|---|---|---|---|---|
| 6xHis | — | 6xHis affinity tag | 46..64 | Fwd | |
|
DNA Sequence
CATCATCATCATCATCAT
Protein Sequence
HHHHHH
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| T7 tag (gene 10 leader) | — | leader peptide from bacteriophage T7 ge… | 67..100 | Fwd | |
|
DNA Sequence
ATGGCTAGCATGACTGGTGGACAGCAAATGGGT
Protein Sequence
MASMTGGQQMG
|
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| Xpress(TM) tag | — | Xpress(TM) epitope tag, including an en… | 103..127 | Fwd | |
|
DNA Sequence
GATCTGTACGACGATGACGATAAG
Protein Sequence
DLYDDDDK
|
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| mKO2 | — | monomeric Kusabira-Orange 2 fluorescent… | 133..787 | Fwd | |
|
DNA Sequence
ATGGTGAGCGTGATCAAGCCCGAGATGAAGATGAGGTACTACATGGACGGCTCCGTCAATGGGCATGAGTTCACAATCGAGGGTGAGGGCACAGGCAGACCTTACGAGGGACATCAGGAGATGACACTGCGCGTCACAATGGCCGAGGGCGGGCCAATGCCTTTCGCCTTCGACCTGGTGTCCCACGTGTTCTGTTACGGCCACAGAGTTTTTACTAAATATCCAGAAGAGATCCCAGACTATTTCAAGCAGGCCTTTCCTGAGGGCCTGTCCTGGGAGAGGTCCCTGGAGTTCGAGGACGGCGGCTCCGCCTCCGTGAGCGCCCACATCAGCCTGAGGGGCAACACCTTCTACCACAAGTCCAAGTTCACCGGCGTGAACTTCCCCGCCGACGGCCCCATCATGCAGAACCAGAGCGTGGACTGGGAGCCCTCCACCGAGAAGATCACCGCCAGCGACGGCGTGCTGAAGGGCGACGTGACCATGTACCTGAAGCTGGAGGGCGGCGGCAACCACAAGTGCCAGATGAAGACCACCTACAAGGCCGCCAAGGAGATCCTGGAGATGCCCGGCGACCACTACATCGGCCACAGGCTGGTGAGGAAGACCGAGGGCAACATCACCGAGCAGGTGGAGGACGCCGTGGCCCACTCC
Protein Sequence
MVSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRVTMAEGGPMPFAFDLVSHVFCYGHRVFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSASVSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMYLKLEGGGNHKCQMKTTYKAAKEILEMPGDHYIGHRLVRKTEGNITEQVEDAVAHS
|
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| hmKO | — | humanized monomeric Kusabira-Orange flu… | 133..787 | Fwd | |
|
DNA Sequence
ATGGTGAGCGTGATCAAGCCCGAGATGAAGATGAGGTACTACATGGACGGCTCCGTCAATGGGCATGAGTTCACAATCGAGGGTGAGGGCACAGGCAGACCTTACGAGGGACATCAGGAGATGACACTGCGCGTCACAATGGCCGAGGGCGGGCCAATGCCTTTCGCCTTCGACCTGGTGTCCCACGTGTTCTGTTACGGCCACAGAGTTTTTACTAAATATCCAGAAGAGATCCCAGACTATTTCAAGCAGGCCTTTCCTGAGGGCCTGTCCTGGGAGAGGTCCCTGGAGTTCGAGGACGGCGGCTCCGCCTCCGTGAGCGCCCACATCAGCCTGAGGGGCAACACCTTCTACCACAAGTCCAAGTTCACCGGCGTGAACTTCCCCGCCGACGGCCCCATCATGCAGAACCAGAGCGTGGACTGGGAGCCCTCCACCGAGAAGATCACCGCCAGCGACGGCGTGCTGAAGGGCGACGTGACCATGTACCTGAAGCTGGAGGGCGGCGGCAACCACAAGTGCCAGATGAAGACCACCTACAAGGCCGCCAAGGAGATCCTGGAGATGCCCGGCGACCACTACATCGGCCACAGGCTGGTGAGGAAGACCGAGGGCAACATCACCGAGCAGGTGGAGGACGCCGTGGCCCACTCC
Protein Sequence
MVSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRVTMAEGGPMPFAFDLVSHVFCYGHRVFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSASVSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMYLKLEGGGNHKCQMKTTYKAAKEILEMPGDHYIGHRLVRKTEGNITEQVEDAVAHS
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