Kohinoor/pRSETb

67770 Synthetic Bacterial Expression

Plasmid Information

Purpose
Kohinoor for bacterial expression
Depositor
Takeharu Nagai
Article
Tiwari et al Nat Methods. 2015 Jun;12(6):515-8. doi:
Insert
Kohinoor
Tags
6xHis
Promoter
T7
Available Since
Sept. 10, 2015
Availability
Available

Sequence Information

Length
870 bp
Topology
linear
Molecule Type
genomic DNA

Features

CDS 4
Label Gene Product Position Strand
6xHis — 6xHis affinity tag 51..69 Fwd
DNA Sequence
CATCATCATCATCATCAT
Protein Sequence
HHHHHH
T7 tag (gene 10 leader) — leader peptide from bacteriophage T7 ge… 72..105 Fwd
DNA Sequence
ATGGCTAGCATGACTGGTGGACAGCAAATGGGT
Protein Sequence
MASMTGGQQMG
Xpress(TM) tag — Xpress(TM) epitope tag, including an en… 108..132 Fwd
DNA Sequence
GATCTGTACGACGATGACGATAAG
Protein Sequence
DLYDDDDK
Kohinoor — fast-switching photoswitchable fluoresc… 141..816 Fwd
DNA Sequence
ATGAGTGTGATTAAACCAGACATGAAGATCAAGCTGCGTATGGAAGGCGCTGTAAATGGACACCCGTTCGCGATTGAAGGAGTTGGCCTTGGGAAGCCTTTCGAGGGAAAACAGAGTATGGACCTTAAAGTCAAAGAAGGCGGACCTCTGCCTTTCGCCTATGACATCTTGACAATGGCCTTCTGTTACGGCAACAGGGTATTCGCCAAATACCCAGAAAATATAGTAGATTATTTCAAGCAGTCGTTTCCTGAGGGCTACTCTTGGGAACGAAGCATGATTTACGAAGACGGGGGCATTTGTATCGCGACAAACGACATAACCCTGGATGGTGACTGTTATATCTATGAAATTCGATTTGATGGTGTGAACTTTCCTGCCAATGGTCCAGTTATGCAGAAGAGGACTGTGAAATGGGAGCCATCCACTGAGAAATTGTATGTGCGTGATGGAGTGCTGAAGAGCGATGGCAACTACGCTCTGTCGCTTGAAGGAGGTGGCCACTACCGATGTGACTCCAAAACTACTTATAAAGCTAAGAAGGTTGTCCAGTTGCCAGACTATCACGATGTGGTCCACCACATTGAGATTAAAAGCCACGACAGAGATTACAGTAATGTTAATCTGCATGAGCATGCCGAAGCGCATTCTGGGCTGCCGAGGCAGGCCAAGTAA
Protein Sequence
MSVIKPDMKIKLRMEGAVNGHPFAIEGVGLGKPFEGKQSMDLKVKEGGPLPFAYDILTMAFCYGNRVFAKYPENIVDYFKQSFPEGYSWERSMIYEDGGICIATNDITLDGDCYIYEIRFDGVNFPANGPVMQKRTVKWEPSTEKLYVRDGVLKSDGNYALSLEGGGHYRCDSKTTYKAKKVVQLPDYHDVVHHIEIKSHDRDYSNVNLHEHAEAHSGLPRQAK