pET28-HADH2

67863 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
bacterial expression of SDR5C1 (HSD17B10), full coding sequence + N-term. His tag (thrombin site)
Depositor
Walter Rossmanith
Article
Holzmann et al Cell. 2008 Oct 31;135(3):462-74. doi: 10
Insert
HADH2 (HSD17B10 Human)
Tags
His
Promoter
T7
Available Since
Aug. 14, 2015
Availability
Available

Sequence Information

Length
1,088 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 3
Label Gene Product Position Strand
6xHis — 6xHis affinity tag 59..77 Fwd
DNA Sequence
CATCATCATCATCATCAC
Protein Sequence
HHHHHH
thrombin site — thrombin recognition and cleavage site 86..104 Fwd
DNA Sequence
CTGGTGCCGCGCGGCAGC
Protein Sequence
LVPRGS
6xHis — 6xHis affinity tag 899..917 Fwd
DNA Sequence
CACCACCACCACCACCAC
Protein Sequence
HHHHHH
terminator 1
Label Gene Product Position Strand
T7 terminator — — 983..1031 Fwd
DNA Sequence
CTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG