MBP-MEK1

68300 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
N-terminal MBP tagged human MEK1 gene for bacterial expression
Depositor
Jesse Rinehart
Article
Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1
Insert
MBP-MEK1 (MAP2K1 Human, Synthetic)
Tags
His and MBP
Available Since
Sept. 10, 2015
Availability
Available

Sequence Information

Length
680 bp
Topology
linear
Molecule Type
genomic DNA

Features

CDS 2
Label Gene Product Position Strand
Factor Xa site — Factor Xa recognition and cleavage site 17..29 Fwd
DNA Sequence
ATCGAGGGAAGG
Protein Sequence
IEGR
TEV site — tobacco etch virus (TEV) protease recog… 29..50 Fwd
DNA Sequence
GAAAATCTGTATTTTCAAGGT
Protein Sequence
ENLYFQG