TriEx-4 DHX36

68368 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
Expresses the N-terminal histidine-tagged DXH36 gene product upon transfection into Rosetta 2 E. coli and induction with IPTG.
Depositor
James Vaughn
Article
Vaughn et al J Biol Chem. 2005 Nov 18;280(46):38117-2
Insert
DHX36 (DHX36 Human)
Tags
Histidine
Promoter
CMV ie enhancer/promoter
Available Since
Sept. 29, 2016
Availability
Available

Sequence Information

Length
913 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 4
Label Gene Product Position Strand
6xHis — 6xHis affinity tag 139..157 Fwd
DNA Sequence
CACCATCACCACCATCAC
Protein Sequence
HHHHHH
S-Tag — affinity and epitope tag derived from p… 166..211 Fwd
DNA Sequence
AAAGAAACCGCTGCTGCGAAATTTGAACGCCAGCACATGGACTCG
Protein Sequence
KETAAAKFERQHMDS
thrombin site — thrombin recognition and cleavage site 226..244 Fwd
DNA Sequence
CTGGTCCCCCGGGGCAGC
Protein Sequence
LVPRGS
enterokinase site — enterokinase recognition and cleavage s… 262..277 Fwd
DNA Sequence
GATGACGACGACAAA
Protein Sequence
DDDDK
promoter 1
Label Gene Product Position Strand
p10 promoter — — 9..119 Fwd
DNA Sequence
GACCTTTAATTCAACCCAACACAATATATTATAGTTAAATAAGAATTATTATCAAATCATTTGTATATTAATTAAAATACTATACTGTAAATTACATTTTATTTACAATC