4YZ8

69955 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
Bacterial expression for structure determination; may not be full ORF
Depositor
Cheryl Arrowsmith
Insert
WHSC1L1:JMC055-F10:C223308 (NSD3 Human)
Tags
His and TEV cleavage site
Promoter
T7-lacO
Available Since
Oct. 7, 2015
Availability
Available

Sequence Information

Length
982 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 3
Label Gene Product Position Strand
6xHis — 6xHis affinity tag 30..48 Rev
DNA Sequence
CACCACCACCACCACCAC
Protein Sequence
HHHHHH
TEV site — tobacco etch virus (TEV) protease recog… 773..794 Rev
DNA Sequence
GAAAACTTGTATTTCCAGGGC
Protein Sequence
ENLYFQG
6xHis — 6xHis affinity tag 806..824 Rev
DNA Sequence
CATCATCATCATCATCAC
Protein Sequence
HHHHHH
promoter 1
Label Gene Product Position Strand
T7 promoter — — 895..914 Rev
DNA Sequence
TAATACGACTCACTATAGG
protein_bind 1
Label Gene Product Position Strand
lac operator — — 870..895 Fwd
DNA Sequence
GGAATTGTTATCCGCTCACAATTCC