pGM202T7

69993 Synthetic Bacterial Expression

Plasmid Information

Purpose
E. coli/Streptomyces expression vector containing the T7-promoter
Depositor
Günther Muth
Article
Muth et al Appl Microbiol Biotechnol. 2018 Nov;102(
Tags
N-terminal and C-terminal His tag encoding sequen…
Available Since
Nov. 13, 2015
Availability
Available

Sequence Information

Length
853 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 4
Label Gene Product Position Strand
6xHis — 6xHis affinity tag 129..147 Fwd
DNA Sequence
CATCATCATCATCATCAT
Protein Sequence
HHHHHH
T7 tag (gene 10 leader) — leader peptide from bacteriophage T7 ge… 150..183 Fwd
DNA Sequence
ATGGCTAGCATGACTGGTGGACAGCAAATGGGT
Protein Sequence
MASMTGGQQMG
Xpress(TM) tag — Xpress(TM) epitope tag, including an en… 186..210 Fwd
DNA Sequence
GATCTGTACGACGATGACGATAAG
Protein Sequence
DLYDDDDK
6xHis — 6xHis affinity tag 228..246 Fwd
DNA Sequence
CATCATCATCATCATCAT
Protein Sequence
HHHHHH
promoter 1
Label Gene Product Position Strand
T7 promoter — — 37..56 Fwd
DNA Sequence
TAATACGACTCACTATAGG
terminator 1
Label Gene Product Position Strand
fd terminator — — 437..486 Rev
DNA Sequence
ACCGATACAATTAAAGGCTCCTTTTGGAGCCTTTTTTTTTGGAGATTTT