| Label | Gene | Product | Position | Strand | |
|---|---|---|---|---|---|
| 6xHis | — | 6xHis affinity tag | 34..52 | Rev | |
|
DNA Sequence
CACCACCACCACCACCAC
Protein Sequence
HHHHHH
|
|||||
| TEV site | — | tobacco etch virus (TEV) protease recog… | 458..479 | Rev | |
|
DNA Sequence
GAAAACCTGTATTTTCAGGGC
Protein Sequence
ENLYFQG
|
|||||
| 6xHis | — | 6xHis affinity tag | 479..497 | Rev | |
|
DNA Sequence
CATCATCATCATCATCAT
Protein Sequence
HHHHHH
|
|||||
| Label | Gene | Product | Position | Strand | |
|---|---|---|---|---|---|
| T7 promoter | — | — | 577..596 | Rev | |
|
DNA Sequence
TAATACGACTCACTATAGG
|
|||||
| lacI promoter | lacI | — | 904..982 | Fwd | |
|
DNA Sequence
GACACCATCGAATGGCGCAAAACCTTTCGCGGTATGGCATGATAGCGCCCGGAAGAGAGTCAATTCAGGGTGGTGAAT
|
|||||
| Label | Gene | Product | Position | Strand | |
|---|---|---|---|---|---|
| lac operator | — | — | 552..577 | Fwd | |
|
DNA Sequence
GGAATTGTTATCCGCTCACAATTCC
|
|||||