pJK681

72271 Synthetic Bacterial Expression

Plasmid Information

Purpose
Produces Synechocystis PCC6803 orf sll1489 (synthetic gene)
Depositor
T. J. Kappock
Insert
hypothetical protein SYNGTS_3005
Tags
His6 and TEV cleavage site
Promoter
T7
Available Since
Feb. 5, 2016
Availability
Available

Sequence Information

Length
907 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 2
Label Gene Product Position Strand
6xHis — 6xHis affinity tag 35..53 Fwd
DNA Sequence
CACCATCATCATCATCAT
Protein Sequence
HHHHHH
TEV site — tobacco etch virus (TEV) protease recog… 77..98 Fwd
DNA Sequence
GAAAATCTGTACTTTCAGTCT
Protein Sequence
ENLYFQS