pTriEx-mCherry-LINXa3-nano

73616 Synthetic Bacterial Expression

Plasmid Information

Purpose
Light-induced nuclear export switch fusion to nano for microscopy in mammalian cells.
Depositor
Brian Kuhlman
Article
Yumerefendi et al Nat Chem Biol. 2016 Jun;12(6):399-401. d
Insert
LINXa3-nano
Tags
mCherry
Available Since
April 27, 2016
Availability
Available

Sequence Information

Length
888 bp
Topology
linear
Molecule Type
genomic DNA

Features

CDS 3
Label Gene Product Position Strand
8xHis — 8xHis affinity tag 42..66 Rev
DNA Sequence
CACCACCATCACCATCACCATCAC
Protein Sequence
HHHHHHHH
HSV tag — HSV (herpes simplex virus) epitope tag 72..105 Rev
DNA Sequence
CAGCCAGAACTCGCCCCGGAAGACCCCGAGGAT
Protein Sequence
QPELAPEDPED
SspB nano — SspB from Haemophilus influenzae, with … 147..477 Rev
DNA Sequence
AGCTCCCCGAAACGCCCTAAGCTGCTGCGTGAATATTACGATTGGCTGGTTGATAACAGCTTTACCCCATATCTGGTGGTGGATGCCACATACCTGGGCGTGAACGTGCCCGTGGAGTATGTGAAAGACGGTCAGATCGTGCTGAATCTGTCTGCAAGTGCGACCGGCAACCTGCAACTGACAAATGATTTTATCCAGTTCAACGCCCGCTTTAAGGGCGTGTCTCGTGAACTGTATATCCCGATGGGTGCCGCTCTGGCCATTTACGCTCGCGAGAACGGCGATGGTGTGATGTTCGAACCAGAAGAAATCTATGACGAGCTGAATATT
Protein Sequence
SSPKRPKLLREYYDWLVDNSFTPYLVVDATYLGVNVPVEYVKDGQIVLNLSASATGNLQLTNDFIQFNARFKGVSRELYIPMGAALAIYARENGDGVMFEPEEIYDELNI