pEMI91

74888 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
Shuffled 9I27 polyprotein with shuffled codons to allow facile sequencing and unique RE for the 9 cassettes for versatile cloning. See Depositor Comments for more information
Depositor
Piotr Marszalek
Article
Scholl et al Biomacromolecules. 2016 Jun 8.
Insert
Titin I27 x 9 (TTN Human)
Tags
His-tag and Strep-tag II
Promoter
T7 promoter
Available Since
June 13, 2016
Availability
Available

Sequence Information

Length
943 bp
Topology
linear
Molecule Type
genomic DNA

Features

CDS 2
Label Gene Product Position Strand
6xHis — 6xHis affinity tag 43..61 Fwd
DNA Sequence
CATCATCATCATCATCAC
Protein Sequence
HHHHHH
thrombin site — thrombin recognition and cleavage site 70..88 Fwd
DNA Sequence
CTGGTGCCGCGCGGCAGC
Protein Sequence
LVPRGS