pPB-CAG-zNanogHD-pgk-hph

74910 Danio rerio Bacterial Expression

Plasmid Information

Purpose
Mammalian expression of zNanog Homeodomain
Depositor
Jose Silva
Article
Theunissen et al Development. 2011 Nov;138(22):4853-65. d
Insert
Nanog
Promoter
CAG
Available Since
May 17, 2016
Availability
Available

Sequence Information

Length
868 bp
Topology
linear
Molecule Type
genomic DNA

Features

polyA_signal 1
Label Gene Product Position Strand
bGH poly(A) signal — — 559..784 Fwd
DNA Sequence
CTGTGCCTTCTAGTTGCCAGCCATCTGTTGTTTGCCCCTCCCCCGTGCCTTCCTTGACCCTGGAAGGTGCCACTCCCACTGTCCTTTCCTAATAAAATGAGGAAATTGCATCGCATTGTCTGAGTAGGTGTCATTCTATTCTGGGGGGTGGGGTGGGGCAGGACAGCAAGGGGGAGGATTGGGAAGACAATAGCAGGCATGCTGGGGATGCGGTGGGCTCTATGG
promoter 1
Label Gene Product Position Strand
lac promoter — — 427..458 Rev
DNA Sequence
TTTACACTTTATGCTTCCGGCTCGTATGTTG
protein_bind 4
Label Gene Product Position Strand
attB1 mutant version of attB — 51..76 Fwd
DNA Sequence
ACAAGTTTGTACAAAAAAGCAGGCT
attB2 mutant version of attB — 334..359 Rev
DNA Sequence
ACCACTTTGTACAAGAAAGCTGGGT
lac operator — — 403..420 Fwd
DNA Sequence
TTGTTATCCGCTCACAA
CAP binding site — — 472..494 Fwd
DNA Sequence
ATGAGTGAGCTAACTCACATTA