pET3a-OSF-CypA

79039 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
Bacterial vector for cloning and propagation in DH5alpha
Depositor
Wesley Sundquist
Article
Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1
Insert
OneSTrEP-FLAG- Cyclophilin A (PPIA Human)
Tags
OneSTrEP-FLAG
Promoter
T7
Available Since
Jan. 30, 2017
Availability
Available

Sequence Information

Length
516 bp
Topology
linear
Molecule Type
genomic DNA

Features

CDS 3
Label Gene Product Position Strand
Strep-Tag II — peptide that binds Strep-Tactin(R), an … 36..60 Fwd
DNA Sequence
TGGAGCCACCCGCAGTTCGAAAAA
Protein Sequence
WSHPQFEK
FLAG — FLAG(R) epitope tag, followed by an ent… 66..90 Fwd
DNA Sequence
GATTACAAGGATGACGATGACAAG
Protein Sequence
DYKDDDDK
HRV 3C site — recognition and cleavage site for human… 105..129 Fwd
DNA Sequence
CTGGAAGTCCTGTTCCAGGGCCCC
Protein Sequence
LEVLFQGP