| Label | Gene | Product | Position | Strand | |
|---|---|---|---|---|---|
| 8xHis | — | 8xHis affinity tag | 30..54 | Fwd | |
|
DNA Sequence
CATCACCATCATCATCATCATCAC
Protein Sequence
HHHHHHHH
|
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| thrombin site | — | thrombin recognition and cleavage site | 63..81 | Fwd | |
|
DNA Sequence
CTGGTTCCGCGTGGTAGT
Protein Sequence
LVPRGS
|
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| SUMO | S. cerevisiae SMT3 (truncated) | cleavable ubiquitin-like protein tag | 81..375 | Fwd | |
|
DNA Sequence
ATGTCGGACTCAGAAGTCAATCAAGAAGCTAAGCCAGAGGTCAAGCCAGAAGTCAAGCCTGAGACTCACATCAATTTAAAGGTGTCCGATGGATCTTCAGAGATCTTCTTCAAGATCAAAAAGACCACTCCTTTAAGAAGGCTGATGGAAGCGTTCGCTAAAAGACAGGGTAAGGAAATGGACTCCTTAAGATTCTTGTACGACGGTATTAGAATCCAAGCTGATCAGACCCCTGAAGATTTGGACATGGAGGATAACGATATTATTGAGGCTCACAGAGAACAGATTGGTGGA
Protein Sequence
MSDSEVNQEAKPEVKPEVKPETHINLKVSDGSSEIFFKIKKTTPLRRLMEAFAKRQGKEMDSLRFLYDGIRIQADQTPEDLDMEDNDIIEAHREQIGG
|
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| TEV site | — | tobacco etch virus (TEV) protease recog… | 378..399 | Fwd | |
|
DNA Sequence
GAAAACTTGTATTTCCAGGGC
Protein Sequence
ENLYFQG
|
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