pMTB2-Avi-Cerulean-Rpl10a

79880 Danio rerio Bacterial Expression

Plasmid Information

Purpose
Beta-actin2 promoter driving Avi-tagged protein containing Cerulean protein fused to zebrafish Rpl10a ribosomal unit (Tryon et al. 2012); flanked by Tol2 sequences
Depositor
Tatjana Sauka-Spengler
Article
Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi
Insert
Cerulean protein fused to zebrafish Rpl10a ribosomal unit (rpl10a Zebrafish)
Tags
Avi
Promoter
Beta-actin2
Available Since
Oct. 11, 2016
Availability
Available

Sequence Information

Length
923 bp
Topology
linear
Molecule Type
genomic DNA

Features

CDS 3
Label Gene Product Position Strand
AviTag(TM) — peptide tag that allows for enzymatic b… 23..68 Fwd
DNA Sequence
GGCCTGAATGACATCTTTGAGGCCCAGAAGATCGAGTGGCATGAG
Protein Sequence
GLNDIFEAQKIEWHE
TEV site — tobacco etch virus (TEV) protease recog… 74..95 Fwd
DNA Sequence
GAGAACCTGTACTTCCAGGGA
Protein Sequence
ENLYFQG
mCerulean — enhanced monomeric variant of CFP (Rizz… 98..815 Fwd
DNA Sequence
ATGGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGTGTCCGGCGAGGGCGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAGTTCATCTGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCCTGACCTGGGGCGTGCAGTGCTTCGCCCGCTACCCCGACCACATGAAGCAGCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAGCGCACCATCTTCTTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGTGAAGTTCGAGGGCGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGACTTCAAGGAGGACGGCAACATCCTGGGGCACAAGCTGGAGTACAACGCCATCAGCGACAACGTCTATATCACCGCCGACAAGCAGAAGAACGGCATCAAGGCCAACTTCAAGATCCGCCACAACATCGAGGACGGCAGCGTGCAGCTCGCCGACCACTACCAGCAGAACACCCCCATCGGCGACGGCCCCGTGCTGCTGCCCGACAACCACTACCTGAGCACCCAGTCCAAGCTGAGCAAAGACCCCAACGAGAAGCGCGATCACATGGTCCTGCTGGAGTTCGTGACCGCCGCCGGGATCACTCTCGGCATGGACGAGCTGTACAAG
Protein Sequence
MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK
RBS 1
Label Gene Product Position Strand
Shine-Dalgarno sequence — — 898..907 Fwd
DNA Sequence
TAAGGAGGT