pGEM-NLS-BirA-2A-mCherry-SV40pA-FKF

79889 Danio rerio Bacterial Expression

Plasmid Information

Purpose
Donor cassette containing HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), mCherry protein and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection gene
Depositor
Tatjana Sauka-Spengler
Article
Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi
Insert
HA-tagged NLS-BirA, 2A, mCherry protein and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
Available Since
Oct. 11, 2016
Availability
Available

Sequence Information

Length
943 bp
Topology
linear
Molecule Type
genomic DNA

Features

CDS 4
Label Gene Product Position Strand
HA — HA (human influenza hemagglutinin) epit… 30..57 Fwd
DNA Sequence
TATGACGTCCCAGATTACGCAAGTTTG
Protein Sequence
YDVPDYASL
HA — HA (human influenza hemagglutinin) epit… 63..90 Fwd
DNA Sequence
TATGATGTTCCTGATTATGCTAGCCTC
Protein Sequence
YDVPDYASL
HA — HA (human influenza hemagglutinin) epit… 96..123 Fwd
DNA Sequence
TACGATGTGCCCGACTATGCCTCACTT
Protein Sequence
YDVPDYASL
SV40 NLS — nuclear localization signal of SV40 (si… 138..159 Fwd
DNA Sequence
CCAAAAAAGAAGAGAAAAGTA
Protein Sequence
PKKKRKV