| Label | Gene | Product | Position | Strand | |
|---|---|---|---|---|---|
| 7xHis | — | 6xHis affinity tag | 136..157 | Fwd | |
|
DNA Sequence
CATCACCATCACCATCACCAT
Protein Sequence
HHHHHHH
|
|||||
| Label | Gene | Product | Position | Strand | |
|---|---|---|---|---|---|
| T5 promoter | — | — | 25..70 | Fwd | |
|
DNA Sequence
TCATAAAAAATTTATTTGCTTTGTGAGCGGATAACAATTATAATA
|
|||||
| Label | Gene | Product | Position | Strand | |
|---|---|---|---|---|---|
| lac operator | — | — | 45..62 | Fwd | |
|
DNA Sequence
TTGTGAGCGGATAACAA
|
|||||
| lac operator | — | — | 77..94 | Fwd | |
|
DNA Sequence
TTGTGAGCGGATAACAA
|
|||||
| attB1 | mutant version of attB | — | 169..194 | Fwd | |
|
DNA Sequence
ACAAGTTTGTACAAAAAAGCAGGCT
|
|||||
| attB2 | mutant version of attB | — | 395..420 | Rev | |
|
DNA Sequence
ACCACTTTGTACAAGAAAGCTGGGT
|
|||||
| Label | Gene | Product | Position | Strand | |
|---|---|---|---|---|---|
| lambda t0 terminator | — | — | 441..536 | Fwd | |
|
DNA Sequence
GACTCCTGTTGATAGATCCAGTAATGACCTCAGAACTCCATCTGGATTTGTTCAGAACGCTCGGTTGCCGCCGGGCGTTTTTTATTGGTGAGAAT
|
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