| Label | Gene | Product | Position | Strand | |
|---|---|---|---|---|---|
| 8xHis | — | 8xHis affinity tag | 199..223 | Rev | |
|
DNA Sequence
CACCACCATCACCATCACCATCAC
Protein Sequence
HHHHHHHH
|
|||||
| HSV tag | — | HSV (herpes simplex virus) epitope tag | 229..262 | Rev | |
|
DNA Sequence
CAGCCAGAACTCGCCCCGGAAGACCCCGAGGAT
Protein Sequence
QPELAPEDPED
|
|||||
| FLAG | — | FLAG(R) epitope tag, followed by an ent… | 301..325 | Rev | |
|
DNA Sequence
GACTACAAGGACGACGACGACAAA
Protein Sequence
DYKDDDDK
|
|||||
| TEV site | — | tobacco etch virus (TEV) protease recog… | 331..352 | Rev | |
|
DNA Sequence
GAAAATCTGTATTTCCAGGGC
Protein Sequence
ENLYFQG
|
|||||
| 6xHis | — | 6xHis affinity tag | 358..376 | Rev | |
|
DNA Sequence
CATCATCATCATCATCAT
Protein Sequence
HHHHHH
|
|||||
| Zdk1 | — | "Zdark" protein, derived from the Z sub… | 394..571 | Rev | |
|
DNA Sequence
ATGGTGGATAACAAATTCAATAAAGAAAAGACGCGTGCCGGTGCGGAAATCCATTCTCTGCCAAACCTGAATGTTGAGCAGAAGTTTGCCTTCATCGTGAGCCTGTTTGATGATCCATCTCAGAGCGCCAATCTGCTGGCCGAAGCCAAAAAACTGAACGATGCCCAGGCCCCAAAA
Protein Sequence
MVDNKFNKEKTRAGAEIHSLPNLNVEQKFAFIVSLFDDPSQSANLLAEAKKLNDAQAPK
|
|||||
| VC155 | — | C-terminal fragment of mVenus for use i… | 589..841 | Rev | |
|
DNA Sequence
GACAAGCAGAAGAACGGCATCAAGGCAAACTTCAAGATCCGCCACAACATCGAGGACGGCGGCGTGCAGCTCGCCGACCACTACCAGCAGAACACCCCCATCGGCGACGGCCCCGTGCTGCTGCCCGACAACCACTACCTGAGCTACCAGTCCAAACTGAGCAAAGACCCCAACGAGAAGCGCGATCACATGGTCCTGCTGGAGTTCGTGACCGCCGCCGGGATCACTCTCGGCATGGACGAGCTGTACAAG
Protein Sequence
DKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK
|
|||||