pET28-mTDGa.0

81050 Mus musculus Bacterial Expression

Plasmid Information

Purpose
bacterial expression of murine TDG
Depositor
Primo Schaer
Article
Weber et al Nat Commun. 2016 Mar 2;7:10806. doi: 10.
Insert
TDG (Tdg Mouse)
Tags
6HIS
Promoter
T7
Available Since
Aug. 19, 2016
Availability
Available

Sequence Information

Length
847 bp
Topology
linear
Molecule Type
genomic DNA

Features

CDS 2
Label Gene Product Position Strand
6xHis — 6xHis affinity tag 46..64 Fwd
DNA Sequence
CATCATCATCATCATCAC
Protein Sequence
HHHHHH
thrombin site — thrombin recognition and cleavage site 73..91 Fwd
DNA Sequence
CTGGTGCCGCGCGGCAGC
Protein Sequence
LVPRGS