pSc-His6-Sem1Sc — PlasmidDB

Plasmid Information

Purpose

pGREG600-His6-Sem1Sc, Expresses the His6-Sem1 in yeast

Depositor

Gali Prag

Article

Levin-Kravets et al Nat Methods. 2016 Nov;13(11):945-952. do

Insert

Sem 1 (SEM1 Budding Yeast)

Length

927 bp

Topology

linear

Molecule Type

genomic DNA

CDS 2

Label	Gene	Product	Position	Strand
Factor Xa site	—	Factor Xa recognition and cleavage site	30..42	Rev
DNA Sequence ATTGAAGGCCGA Protein Sequence IEGR
6xHis	—	6xHis affinity tag	269..287	Rev
DNA Sequence CATCACCATCATCACCAC Protein Sequence HHHHHH

promoter 2

Label	Gene	Product	Position	Strand
GAL1 promoter	S. cerevisiae GAL1	—	383..825	Rev
DNA Sequence CGGATTAGAAGCCGCCGAGCGGGTGACAGCCCTCCGAAGGAAGACTCTCCTCCGTGCGTCCTCGTCTTCACCGGTCGCGTTCCTGAAACGCAGATGTGCCTCGCGCCGCACTGCTCCGAACAATAAAGATTCTACAATACTAGCTTTTATGGTTATGAAGAGGAAAAATTGGCAGTAACCTGGCCCCACAAACCTTCAAATGAACGAATCAAATTAACAACCATAGGATGATAATGCGATTAGTTTTTTAGCCTTATTTCTGGGGTAATTAATCAGCGAAGCGATGATTTTTGATCTATTAACAGATATATAAATGCAAAAACTGCATAACCACTTTAACTAATACTTTCAACATTTTCGGTTTGTATTACTTCTTATTCAAATGTAATAAAAGTATCAACAAAAAATTGTTAATATACCTCTATACTTTAACGTCAAGGAG
T3 promoter	—	—	864..883	Rev
DNA Sequence AATTAACCCTCACTAAAGG

protein_bind 1

Label	Gene	Product	Position	Strand
UAS	—	—	707..825	Fwd
DNA Sequence CGGAGCAGTGCGGCGCGAGGCACATCTGCGTTTCAGGAACGCGACCGGTGAAGACGAGGACGCACGGAGGAGAGTCTTCCTTCGGAGGGCTGTCACCCGCTCGGCGGCTTCTAATCCG