pmcsg7-mCRT(WT)

83505 Mus musculus Bacterial Expression

Plasmid Information

Purpose
Bacterial expression of Calreticulin
Depositor
Malini Raghavan
Article
Del Cid et al J Biol Chem. 2010 Feb 12;285(7):4520-35.
Insert
CALR (Calr Mouse)
Tags
His
Promoter
T7
Available Since
Oct. 12, 2016
Availability
Available

Sequence Information

Length
950 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 2
Label Gene Product Position Strand
6xHis — 6xHis affinity tag 51..69 Fwd
DNA Sequence
CACCATCATCATCATCAT
Protein Sequence
HHHHHH
TEV site — tobacco etch virus (TEV) protease recog… 93..114 Fwd
DNA Sequence
GAGAACCTGTACTTCCAATCC
Protein Sequence
ENLYFQS