hsDCX-EGFP-pHAT

83918 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
human Doublecortin, N-terminal 6-His tag, C-terminal EGFP-Strep-tag II
Depositor
Gary Brouhard
Article
Bechstedt et al Dev Cell. 2012 Jul 17;23(1):181-92. doi:
Insert
Doublecortin isoform b (DCX NM_178152.2, Human)
Tags
6-His and EGFP+Strep-tag II
Available Since
Dec. 8, 2016
Availability
Available

Sequence Information

Length
1,055 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 2
Label Gene Product Position Strand
6xHis — 6xHis affinity tag 53..71 Fwd
DNA Sequence
CATCACCATCACCATCAC
Protein Sequence
HHHHHH
HRV 3C site — recognition and cleavage site for human… 77..101 Fwd
DNA Sequence
CTGGAAGTTCTGTTCCAGGGGCCC
Protein Sequence
LEVLFQGP