p3R112C/T180CBM3

85090 Synthetic Bacterial Expression

Plasmid Information

Purpose
Expresses PCNA3 variant (R112C/T180C) fused to P450 BM3 variant (A74G/C773S/C810S) in E. coli
Depositor
Teruyuki Nagamune
Article
Tan et al Angew Chem Int Ed Engl. 2016 Oct 26. doi
Insert
The R112C/T180C variant of PCNA3 fused to P450 BM3
Tags
His6 tag
Mutation
R112C/T180C in PCNA3, A74G/C773S/C810S in P450BM3
Promoter
T7 promoter
Available Since
Dec. 12, 2016
Availability
Available

Sequence Information

Length
1,012 bp
Topology
linear
Molecule Type
genomic DNA

Features

CDS 2
Label Gene Product Position Strand
6xHis — 6xHis affinity tag 58..76 Fwd
DNA Sequence
CATCATCATCATCATCAC
Protein Sequence
HHHHHH
thrombin site — thrombin recognition and cleavage site 85..103 Fwd
DNA Sequence
CTGGTGCCGCGCGGCAGC
Protein Sequence
LVPRGS