pGS-3

85094 Synthetic Bacterial Expression

Plasmid Information

Purpose
Host vector to fuse a gene at the N-terminus of PCNA3
Depositor
Teruyuki Nagamune
Article
Tan et al Angew Chem Int Ed Engl. 2016 Oct 26. doi
Insert
DNA polymerase sliding clamp 3
Tags
His6 tag
Promoter
T7 promoter
Available Since
Nov. 29, 2016
Availability
Available

Sequence Information

Length
913 bp
Topology
linear
Molecule Type
genomic DNA

Features

CDS 2
Label Gene Product Position Strand
6xHis — 6xHis affinity tag 55..73 Fwd
DNA Sequence
CATCATCATCATCATCAC
Protein Sequence
HHHHHH
thrombin site — thrombin recognition and cleavage site 82..100 Fwd
DNA Sequence
CTGGTGCCGCGCGGCAGC
Protein Sequence
LVPRGS