pF714-pET28a-Hs-FL-METTL20-NHis wt

85115 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
expression of recombinant human full-length METTL20 wt with N-terminal 6xHis-tag
Depositor
Pal Falnes
Article
Malecki et al J Biol Chem. 2015 Jan 2;290(1):423-34. d
Insert
human full-length METTL20 (METTL20 Human)
Tags
6xHis
Promoter
T7
Available Since
Nov. 12, 2018
Availability
Available

Sequence Information

Length
919 bp
Topology
linear
Molecule Type
genomic DNA

Features

CDS 2
Label Gene Product Position Strand
6xHis — 6xHis affinity tag 52..70 Fwd
DNA Sequence
CATCATCATCATCATCAC
Protein Sequence
HHHHHH
thrombin site — thrombin recognition and cleavage site 79..97 Fwd
DNA Sequence
CTGGTGCCGCGCGGCAGC
Protein Sequence
LVPRGS