HOXB3 (human) HIS-tag pET

8524 Homo sapiens Bacterial Expression

Plasmid Information

Depositor
Corey Largman
Article
Shen et al Nucleic Acids Res 1996 Mar 1;24(5):898-9
Insert
HOXB3 (HOXB3 Human)
Tags
His and T7
Mutation
Contains 5 additional amino acids before ATG from…
Available Since
May 30, 2006
Availability
Available

Sequence Information

Length
849 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 3
Label Gene Product Position Strand
thrombin site — thrombin recognition and cleavage site 4..22 Fwd
DNA Sequence
CTGGTGCCGCGCGGCAGC
Protein Sequence
LVPRGS
T7 tag (gene 10 leader) — leader peptide from bacteriophage T7 ge… 25..58 Fwd
DNA Sequence
ATGGCTAGCATGACTGGTGGACAGCAAATGGGT
Protein Sequence
MASMTGGQQMG
6xHis — 6xHis affinity tag 310..328 Fwd
DNA Sequence
CACCACCACCACCACCAC
Protein Sequence
HHHHHH
rep_origin 1
Label Gene Product Position Strand
f1 ori — — 478..838 Fwd
DNA Sequence
ACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCGGGGGCTCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGATTAGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACCCCTATCTCGGTCTATTCTTTTGATTTA
terminator 1
Label Gene Product Position Strand
T7 terminator — — 394..442 Fwd
DNA Sequence
CTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG