3521 pET15b Bid p15

8782 Mus musculus Bacterial Expression

Plasmid Information

Depositor
Stanley Korsmeyer
Article
Wei et al Genes Dev 2000 Aug 15;14(16):2060-71.
Insert
Bid p15 (Bid Mouse)
Tags
His
Available Since
July 6, 2005
Availability
Available

Sequence Information

Length
743 bp
Topology
linear
Molecule Type
genomic DNA

Features

CDS 2
Label Gene Product Position Strand
6xHis — 6xHis affinity tag 40..58 Fwd
DNA Sequence
CATCATCATCATCATCAC
Protein Sequence
HHHHHH
thrombin site — thrombin recognition and cleavage site 67..85 Fwd
DNA Sequence
CTGGTGCCGCGCGGCAGC
Protein Sequence
LVPRGS
terminator 1
Label Gene Product Position Strand
T7 terminator — — 577..625 Fwd
DNA Sequence
CTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG