| Label | Gene | Product | Position | Strand | |
|---|---|---|---|---|---|
| mKO2 | — | monomeric Kusabira-Orange 2 fluorescent… | 0..4123 | Fwd | |
|
DNA Sequence
GTGAGCGTGATCAAGCCCGAGATGAAGATGAGGTACTACATGGACGGCTCCGTCAATGGGCATGAGTTCACAATCGAGGGTGAGGGCACAGGCAGACCTTACGAGGGACATCAGGAGATGACACTGCGCGTCACAATGGCCGAGGGCGGGCCAATGCCTTTCGCCTTCGACCTGGTGTCCCACGTGTTCTGTTACGGCCACAGAGTTTTTACTAAATATCCAGAAGAGATCCCAGACTATTTCAAGCAGGCCTTTCCTGAGGGCCTGTCCTGGGAGAGGTCCCTGGAGTTCGAGGACGGCGGCTCCGCCTCCGTGAGCGCCCACATCAGCCTGAGGGGCAACACCTTCTACCACAAGTCCAAGTTCACCGGCGTGAACTTCCCCGCCGACGGCCCCATCATGCAGAACCAGAGCGTGGACTGGGAGCCCTCCACCGAGAAGATCACCGCCAGCGACGGCGTGCTGAAGGGCGACGTGACCATGTACCTGAAGCTGGAGGGCGGCGGCAACCACAAGTGCCAGATGAAGACCACCTACAAGGCCGCCAAGGAGATCCTGGAGATGCCCGGCGACCACTACATCGGCCACAGGCTGGTGAGGAAGACCGAGGGCAACATCACCGAGCAGGTGGAGGACGCCGTGGCCCACTCC
Protein Sequence
VSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRVTMAEGGPMPFAFDLVSHVFCYGHRVFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSASVSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMYLKLEGGGNHKCQMKTTYKAAKEILEMPGDHYIGHRLVRKTEGNITEQVEDAVAHS
|
|||||
| hmKO | — | humanized monomeric Kusabira-Orange flu… | 0..4123 | Fwd | |
|
DNA Sequence
GTGAGCGTGATCAAGCCCGAGATGAAGATGAGGTACTACATGGACGGCTCCGTCAATGGGCATGAGTTCACAATCGAGGGTGAGGGCACAGGCAGACCTTACGAGGGACATCAGGAGATGACACTGCGCGTCACAATGGCCGAGGGCGGGCCAATGCCTTTCGCCTTCGACCTGGTGTCCCACGTGTTCTGTTACGGCCACAGAGTTTTTACTAAATATCCAGAAGAGATCCCAGACTATTTCAAGCAGGCCTTTCCTGAGGGCCTGTCCTGGGAGAGGTCCCTGGAGTTCGAGGACGGCGGCTCCGCCTCCGTGAGCGCCCACATCAGCCTGAGGGGCAACACCTTCTACCACAAGTCCAAGTTCACCGGCGTGAACTTCCCCGCCGACGGCCCCATCATGCAGAACCAGAGCGTGGACTGGGAGCCCTCCACCGAGAAGATCACCGCCAGCGACGGCGTGCTGAAGGGCGACGTGACCATGTACCTGAAGCTGGAGGGCGGCGGCAACCACAAGTGCCAGATGAAGACCACCTACAAGGCCGCCAAGGAGATCCTGGAGATGCCCGGCGACCACTACATCGGCCACAGGCTGGTGAGGAAGACCGAGGGCAACATCACCGAGCAGGTGGAGGACGCCGTGGCCCACTCC
Protein Sequence
VSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRVTMAEGGPMPFAFDLVSHVFCYGHRVFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSASVSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMYLKLEGGGNHKCQMKTTYKAAKEILEMPGDHYIGHRLVRKTEGNITEQVEDAVAHS
|
|||||
| AmpR | bla | beta-lactamase | 1798..2659 | Fwd | |
|
DNA Sequence
ATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAA
Protein Sequence
MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW
|
|||||
| 6xHis | — | 6xHis affinity tag | 3765..3783 | Fwd | |
|
DNA Sequence
CATCATCATCATCATCAT
Protein Sequence
HHHHHH
|
|||||
| T7 tag (gene 10 leader) | — | leader peptide from bacteriophage T7 ge… | 3786..3819 | Fwd | |
|
DNA Sequence
ATGGCTAGCATGACTGGTGGACAGCAAATGGGT
Protein Sequence
MASMTGGQQMG
|
|||||
| Xpress(TM) tag | — | Xpress(TM) epitope tag, including an en… | 3822..3846 | Fwd | |
|
DNA Sequence
GATCTGTACGACGATGACGATAAG
Protein Sequence
DLYDDDDK
|
|||||
| Label | Gene | Product | Position | Strand | |
|---|---|---|---|---|---|
| AmpR promoter | bla | — | 1693..1798 | Fwd | |
|
DNA Sequence
CGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGT
|
|||||
| T7 promoter | — | — | 3673..3692 | Fwd | |
|
DNA Sequence
TAATACGACTCACTATAGG
|
|||||
| Label | Gene | Product | Position | Strand | |
|---|---|---|---|---|---|
| f1 ori | — | — | 1212..1668 | Fwd | |
|
DNA Sequence
ACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCGGGGGCTCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGATTAGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACCCTATCTCGGTCTATTCTTTTGATTTATAAGGGATTTTGCCGATTTCGGCCTATTGGTTAAAAAATGAGCTGATTTAACAAAAATTTAACGCGAATTTTAACAAAATATTAACGCTTACAATTT
|
|||||
| ori | — | — | 2829..3418 | Fwd | |
|
DNA Sequence
TTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAA
|
|||||
| Label | Gene | Product | Position | Strand | |
|---|---|---|---|---|---|
| T7 terminator | — | — | 1068..1116 | Fwd | |
|
DNA Sequence
CTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG
|
|||||